Combinational Domain Hunting

Combinational Domain Hunting (CDH) identifies segments of a target gene that express—in high yield—protein constructs which are correctly folded, stable, and soluble. The main elements of the CDH technology are:

• Design of a synthetic gene, optimised for expression in E.coli.
• Random fragmentation of the gene using our patented method.
• Cloning of the tagged gene fragments into E.coli.
• Expression of a library of protein constructs, and screening of these for the desired characteristics.
• Confirmation of the desired functionality in “hit” fragments.

CDH is protected by granted European patent EP1442134, with further US and worldwide patents pending.

Recent developments to CDH have allowed us to significantly increase our throughput by prosecuting groups of targets as "cassettes". This multiplexed-mode of CDH can allow us to offer clients special terms for multi-target deals. Domainex is also using this increased capacity to work through a "gallery" of CDH targets over the next year or so. Most of the gallery targets are proteins that are known to be difficult to work with, but which have good disease validation. Commercial deals around gallery targets can be developed with customers on an exclusive or non-exclusive basis. If you would like to discuss an existing gallery target with Domainex, or would like to nominate a target for inclusion in the gallery, please contact us.